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Once formed, 5 methylTHF is used by methionine synthase for the vitamin B 12 -dependent conversion of homocysteine to methionine and the formation of THF ( Figure 2 )

Interestingly, in one study, the authors used metformin, which activates AMPK and autophagy, and it was studied in diabetic patients to improve insulin sensitivity and lipid metabolism

Time-lapse images of cells were acquired with the Olympus cellSens Dimension system, consisting of an Olympus Ixplore SpinSR10 spinning disk confocal and a Yokogawa CSU-W1 confocal scanner